Fixed Sample Imaging

The two microscopes grouped as fixed sample imaging are extremely mature technologies that were among the first to be developed for structural studies on a cellular size scale.  Each of these methods does an excellent job with multiply-labeled samples for 2-D or 3-D imaging. In our facility we have: 

  • APLLC DeltaVision deconvolution microscope: This microscope removes out-of-focus blur from images using a computational approach employing an experimentally-measured point spread (blurring) function.  
  • Olympus FV1000 laser point-scanning confocal microscope: Out-of-focus blur from images is removed using an aperture (pinhole) placed in front of the imaging camera which is conjugate to the image focal plane.  The raster-scan point scanning laser method of this instrument was the first confocal method developed for biological imaging.