The two microscopes grouped as fixed sample imaging are extremely mature technologies that were among the first to be developed for structural studies on a cellular size scale.  Each of these methods does an excellent job with multiply-labeled samples for 2-D or 3-D imaging. In our facility we have: 

• DeltaVision Ultra deconvolution microscope: This microscope removes out-of-focus blur from images using a computational approach employing an experimentally-measured point spread (blurring) function.